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Singleron PythoN® Tissue Dissociation System

The Singleron PythoN® Automated Tissue Dissociation System integrates mechanical and enzymatic dissociation. The streamlined workflow quickly dissociates the tissue into high-quality single-cells suspension. Equipped with broad-spectrum sCelLiVE® Tissue Dissociation Solution and disposable PythoN Dissociation Tubes, the system can automatically process tissues to obtain highly viable and high-quality single-cell suspensions. The precise digital controling module enables a quick exploration of the best dissociation conditions for a specific tissue type.

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Singleron PythoN® produces highly viable, high-quality single-cell suspension with high yield.

Singleron PythoN® was used to dissociate murine heart, testicle, liver, lung, kidney and spleen tissues, which results in high-quality single cell suspensions with high cell viability and cell yield.

 

Singleron PythoN® dissociates tissues with high reproducibility.

Muirne liver tissues were processed in triplicates using Singleron PythoN®. Single cell suspensions with >85% cell activity were obtained. Cell clustering and cell type ratios were consistent across the three replicates, showing the high stability and reproducibility of the Singleron PythoN® Tissue Dissociator.

 

Singleron PythoN® is also suitable for low-input samples, even for tissue samples as low as 10mg. 

In clinical settings, in order to detect the pathological characteristics of specific tissues, needle biopsy is often required. The low weight of the obtained needle biopsies is often a challenge for single-cell sequencing workflow (the weight of a single sample is about 20-40 mg). Here, we used Singleron PythoN® to process 14 mg and 33 mg mouse liver tissues, and achieved high cell viability and yield accordingly. This enabled applying clinical needle biopsies to single cell sequencing workflows. 

 

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