The immune system is highly complicated. In addition to the traditional classification of immune cell types, the strength of immune cell functions and the states of activation, inhibition, and exhaustion will have a great impact on immune functions. These effects are not only related to infectious diseases and autoimmune diseases, but also to the immune reactions after transplantation. High-throughput single-cell sequencing can not only profile the types and status of immune cells, but also analyze the complex interaction between the activation or inhibition of cells, providing a powerful tool for immunological research.
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