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A new wave of customizable solutions to achieve advanced levels of precision in single cell research

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A new wave of customizable solutions to achieve advanced levels of precision in single cell research

Recently, single cell transcriptome sequencing technology has rapidly developed to provide an effective method for researchers to study the heterogeneity of cells; to understand molecular mechanisms and developmental processes; and to investigate different disease states at a single cell resolution. The growing interest to conduct research at a single cell level, has led to rapid technological advances in single cell sequencing methods to evolve from low to high cell throughput and progression from single cell transcriptomics to single cell multi-omics. The recent integration of microfluidic technology for high throughput single cell transcriptome sequencing has enhanced the capacity to simultaneously obtain data from a significant number of cells. Singleron Biotechnologies provides flexible solutions to customize your single cell research to achieve multi-omics analysis.

Application 1: Customized tissue dissociation procedures

The Singleron PythoN® tissue dissociation instrument, fully compatible with our sCelLive® tissue dissociation solution, is equipped with specialized Singleron PythoN® dissociation units, to efficiently automate the preparation of single cell suspensions to capture high quality and high viability single cell suspensions. Operating with a precise digital parameter adjustment module, Singleron PythoN® can be customized to enhance the dissociation procedure for specific and difficult tissues.

Figure 1. Singleron PythoN® automated tissue dissociation system. Equipped with broad-spectrum sCelLive Tissue dissociation mix, disposable Singleron PythoN® dissociation units and the Singleron PythoN® tissue dissociator offers a streamlined workflow to quickly dissociate tissue into high quality single cell suspensions.

Application 2: Large well SCOPE-chip®

The ability to make precise measurements of single cells to unlock and answer biological questions are potentially hindered by the morphological diversity of cells. Previously, the irregular shape and large size of certain cells, for example, cardiomyocytes, squamous epithelial cells, and plant protoplasts, has limited the capture rate to be able to measure their biological status. To overcome this complication, our SCOPE-chip® has been optimized to specifically capture cells with a cell diameter greater than 40 µm, progressing single cell technology towards a wider field of application.

Application 3: Customize single cell sequencing to target specific genes

Deciphering the connection between genotype and phenotype at a single cell level can potentially unlock applications ranging from cell lineage tracing to analyzing the sub-clonal architecture in tumours. The phenotypic diversity between subclones may contribute to drug sensitivity, diverse disease mechanisms and distinct evolutionary paths of different driver mutations, therefore, targeting specific genes in single cell sequencing can aid in addressing these factors. Singleron’s FocuSCOPE® is a high-throughput single cell multi-omics sequencing solution to detect both target sequences of interest and transcriptome simultaneously from the same single cell. Our technology is based on unique barcoding beads conjugated with oligos to efficiently capture both whole transcripts and targeted gene transcripts. Reaction chemistry is utilized for simultaneous amplification and library construction of both whole transcript and target region sequences, to improve the detection rate of target sequences.

Figure 2. Singleron offers custom probe design on our uniquely designed AccuraCell beads to target up to 5 regions of interest, to be capture simultaneously.

FocuSCOPE® has successfully been used for single cell analysis of both gene expression profiles and point mutations, fusion genes, or intracellular viral sequences simultaneously. In addition to this, the barcoding oligos of FocuSCOPE® can be customized to target up to 5 target sequences of your choice.

Figure 3. FocuSCOPE® targeted high throughput single cell sequencing workflow

FocuSCOPE® targeted high throughput single cell sequencing application:

1. Detection of regions containing high frequency of mutations

2. Fusion gene targeted detection

3. Non-polyadenylated RNA detection

If you are interested in any of these products or want to acquire more details on these customizable solutions, please consult our local sales or send an email to info@singleronbio.com.

A post by Singleron team

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