GEXSCOPE® Single Cell RNA Library Kit GEXSCOPE® Single Nucleus RNA Library Kit GEXSCOPE® Microbial Single Cell RNA Library Kit HD (Yeast) GEXSCOPE® Single Cell V(D)J Kit DynaSCOPE® Single Cell Dynamics RNA Library Kit FocuSCOPE® Single Cell Targeted Capture Kit sCircle® Single Cell Full Length Immunoreceptor Library Kit CLindex® Sample Multiplexing Kit sCelLiVE® Tissue to Living Single Cell Suspension Kit AccuraCode® RNA Library Construction Kit
sCelLiVE® Tissue to Living Single Cell Suspension Kit, comprising two separate reagents - sCelLiVE® Tissue Preservation Buffer and sCelLiVE® Tissue Dissociation Master Mix, is an all-in-one and ready-to-use solution from various fresh and solid tissue types to single cell suspension with a viability of typically >90%. sCelLiVE® kit ensures dissociation into single cell suspensions without the requirement of protocol optimization for the vast majority of tissue types and reagent preparation.
• Single cell sequencing
• Flow Cytometry
• Cell sorting
• Single cell culture
• Single cell proteomics
• Versatility – can be easily applied to all fresh and solid tissues (e.g. surgically resected samples and needle biopsies)
• Easy and fast workflow – a simple and straightforward workflow up to 1 hour
• One-for-all solution – one product effective on a large variety of tissue types and species
• Secure high-quality results – including prevention of rare cell type loss
sCelLiVETM Tissue Preservation Solution
sCelLiVE® Tissue Preservation Buffer efficiently simulates the physiological environment and ensures high cell viability over a 72-hour time period.
Figure: Viability of cells using sCelLiVE® Tissue Preservation Solution with brain tissues collected freshly or stored at 4ºC for 24 and 72 hours. Approximately 90% of the cells from the tissue stored in the Preservation Solution were still alive after 72 hours storage.
Figure: The single cell sequencing of fresh tissue or the same tissue stored for 24 or 72 hours in sCelLiVE® Tissue Preservation Solution was performed. The same cellular compositions were identified in all three samples by t-SNE plots analysis. The resulting gene expression pattern is not affected.
sCelLiVE Dissociation Master Mix enzymatically dissociates various samples. This unique Dissociation Master Mix is verified on more than 300 sample types from both human and mice.
Figure: Comparison of sCelLiVE® Tissue Dissociation Master Mix to other protocols on dissociation of glioblastoma samples: a homebrew protocol from literature and a commercial product from Supplier M. Cell suspension generated with sCelLiVE® Tissue Dissociation Master Mix had the highest cellular viability. The proprietary formulation does not affect cell viability.
Simple Automated Tissue Dissociation Process with High Cell Yield
sCelLiVE® Dissociation Master Mix ensures high cell yield. Total cell yield of diverse clinical samples evaluated using the PythoN®, an automated 8-channel tissue dissociation system. The results are shown in the number of cell yield/mg of the tissue.
Enabling analysis of Rare Cell Populations
Using sCelLiVE® Tissue to Living Single Cell Suspension Kit, the high viability of cells ensures a proper analysis of all cell types, even those prone to degradation such as neutrophils and also derived from a very low sample amount.
Figure: Ultra-high throughput single cell sequencing of a complete mouse embryo using High-Density chip GEXSCOPE® Single Cell RNA Library Kit is shown here. The analysis detected gene expression in 42,641 cells from the same sample and identified a rare cell population accounting for as little as 0.15% of total cells.
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