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Single Cell Partitioning That Is Gentle on Your Sample

Discover the SCOPE-chip® micro-well microfluidics system, designed for gentle single cell partitioning. Using gravity to guide cells into individual wells, SCOPE-chip ensures robust performance, even with sensitive or fragile cell types.

Technology Highlights

Our SCOPE-chip technology-based kits cover the entire single cell sample processing workflow—from tissue preparation, single-cell partitioning, and cell barcoding to nucleic acid amplification and library construction.


cell handling that does not bias against sensitive cell types


performance trusted by 2900+ labs worldwide


Captures 500 – 30,000 cells or nuclei per chip

Low doublet rate

with our instrument-free workflow or fully automated solutions


your workflow with integrated automation options

The patented SCOPE-chip® (Single Cell Omics Preparation Entity) technology is developed by Singleron in collaboration with Yale University.

Singleron has more than 80 patents and numerous patent applications. Our innovations span instrument and microfluidic system design, single cell amplification, library construction, and bioinformatic analysis algorithms.

How does the SCOPE-chip work?

Cells are partitioned into hundreds of thousands of microwells on the chip following the Poisson distribution

An excess of Barcoding Beads is added to the microwells of the chip; the diameter of the beads versus that of the microwells ensuring that only one Barcoding Bead falls into each microwell

After cell lysis, Barcoding Beads, each with an unique cell label (Barcode), capture mRNA by binding to the poly (A) tail on the mRNA

Barcoding Beads are subsequently collected from the microwell chip using a P200 pipette

The rest of library preparation steps including reverse transcription (RT), amplification, and library construction are done in standard Eppendorf tubes

The sequencing library is compatible with Illumina sequencing instruments, and can also be run on Pacbio and MGI sequencing platforms with minor modifications

SCOPE-chip loading: easy & instrument-free


As our chip is an open system, it allows some changes to meet the needs of each experiment. In this regard, a different buffer can be used (for example W5 buffer for protoplasts) when it doesn’t contain metal chelator agents that might interfere with the reverse transcription (such as EDTA), and it must be RNAse free. In this case, we recommend following these steps:

  1. Wash the chip with your own buffer prior to cell loading.
  2. Load the cells, which are in suspension in your own buffer.
  3. Wash the chip with PBS to get rid of the excess cells that are not within microwells, following the handbook instructions.
  4. Load the beads (resuspended in 60ul of PBS), following the handbook instructions.
  5. Add the lysis buffer and continue with the instructions as described in the handbook.

To ensure the best handling of the SCOPE-chip, please use one of our recommended pipette tips:

  • Biofil PMT231200
  • Eppendorf cat #0030078551
  • VWR cat #732-3701

Due to the design of the chip, background noise from ambient RNA is very low. In addition, no spillover of RNA between wells was detected since wells containing only the beads had no RNA detected above the ambient level cutoff.

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