The AccuraCode® TCR Library Construction Kit enables efficient, accurate, and scalable exploration of T-cell repertoires. It simultaneously profiles TCRs from multiple samples and facilitates comprehensive analysis of T-cell clonotypes, gene usage, V(D)J recombination patterns, and diversity.
For this purpose CCRF-CEM cells were used, a cell line derived from an acute lymphoblastic leukemia (ALL) patient and known to consist of one clonotype only T-cell lymphoblasts. For the sensitivity testing, PBMCs were spiked with varying fractions of CCRF-CEM cells.
(A) Schematic overview of the experimental setup and workflow. PBMCs and CCRF-CEM cells were mixed at different proportions. Lysis was performed on plate prior to library generation. (B) Tested cell compositions. (C) AccuraCode TCR accurately detects the proportion of CCRF cells from the mixture at 1-20% CCRF frequency.
Plate-based workflow
for human and mouse samples
Instrument-independent workflow
The probes will capture all transcripts with poly-A tail. An enrichment of the CDR3 region by PCR will be performed afterwards.
No. As the kit’s goal is to focus on TCR analysis, a parallel construction of transcriptomic library is not required.
The recommended input for cells is 10k-100k per well. It is not recommended to go under 5k cell per well.
Currently, the kit supports tissues originated from human and mouse. The cells can be fresh or frozen (less recommended). Alternatively, RNA (20-100ng/well) or whole blood (>20µL) can also be used.
The current workflow enables the simultaneous preparation of up to 96 ready-to-sequence libraries from cell suspension within a timeframe of 11 hours.
Part of the workflow involves the use of 96-well plate(s). Therefore, suitable magnet and thermomixer for the plate are required. We highly suggest the use of a multichannel pipette.
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