Focus on genes that matter! The FocuSCOPE® Single Cell Targeted Capture Kit utilizes targeted customized probes for simultaneous whole transcriptome sequencing and detection of mutations, gene fusions, and viral sequences from the same single cells.
The core technology of FocuSCOPE® is centered around specially designed Barcoding Beads with both customizable probes and poly-T probes that capture poly-adenylated mRNA and your specific transcripts of interest. During the workflow, cDNA is split, and two different libraries are prepared which can be combined for sequencing.
WT1, KRAS, IDH1/2, TP53, BCR-ABL1, PML-RARA
EGFR, KRAS, PIK3CA, BRAF, TP53
DNMT3A, TET2, ASXL1, JAK2, TP53
EBNA1, EBNA2 , EBERs , ZEBRA
16S rRNA (V2, V4, V5, V6, V8 hypervariable region)
A xenografted tumor model, derived from the NB4 cell line harboring the PML-RARA fusion gene t (15;17), was utilized. Clustering data unveiled a notably elevated detection rate of the PML-RARA fusion gene in libraries constructed with our targeted FocuSCOPE beads in combination with poly(dT) probes, compared to libraries constructed with poly(dT) probes alone. Data show increased PML-RARA fusion gene detection rate using FocuSCOPE targeted probes.
The Raji cell line, which carries the latent Epstein-Barr Virus, was employed alongside A549 cell lines as a control. Libraries were prepared using either FocuSCOPE® EBV beads (comprising EBV-targeted probes and poly(dT) probes) or beads with poly(dT) probes alone. Sequencing data revealed a higher detection rate for EBV in the group utilizing our FocuSCOPE® EBV beads.
In this cell-line-derived xenograft (CDX) tumor model, NB4 cells, a cell line harboring a single nucleotide variant of TP53 (R248Q) and the PML-RARA fusion gene was subcutaneously injected into immunodeficient mice. Libraries were generated using FocuSCOPE Custom Tag Barcoding Beads or poly-T beads. Sequencing data revealed a higher detection rate for the SNV of TP53 in the group utilizing our FocuSCOPE® SNV beads.
fully customizable (up to 200bp target region) | pre-validated panels (Blood cancer, CHIP, EBV and lung cancer)
choose between SD and HD chip depending on required capture rate
Full instruments-free workflow
PythoN: tissue dissociation NEO: single cell processing
I have been very happy with Singleron’s technology. The workflow was straightforward, worked smoothly, and generated very good-quality data. The microwell-based method proved extremely robust, even with delicate samples such as infected organoids that had been dissociated and sorted. Another major advantage is that the procedure can be performed at the bench, without specialised instruments or dedicated facilities. The customisation service and technical support were exceptional. The team helped tailor the kit to my research needs, provided support throughout the process, and even ran pilot experiments to optimise the approach. Overall, I had an excellent experience, both with the technology itself and with the Singleron team. I would particularly recommend it to researchers looking for a straightforward, bench-based single-cell workflow backed by responsive, knowledgeable, and highly supportive technical assistance.
Two libraries are generated (whole transcriptome + enrichment library).
Beads consist of both PolyT and target probes. There is a tradeoff. We recommend not going more than 10 to sufficiently capture the whole transcriptome.
For FocuSCOPE, we recommend Paired-End (PE) 150. Sequencing Depth: 20,000 – 50,000 read pairs per cell for the whole transcriptome library and 5,000 reads per cell for the enrichment library.
Yes, it can detect deletions.
The length of our probes is ~25-30 bases.
The kit is suitable for human PBMC and bone marrow.
It is possible to use cryopreserved cells.
Start your single cell journey today! Contact us for a quote or assistance in planning your next experiment.