Investigate T-cell or B-cell receptor gene expression, together with whole transcriptome expression at single-cell level.
Combined transcriptomics and TCR/BCR repertoire profiling. Start from fresh tissue or cryopreserved cells. Barcoding beads capture both poly-adenylated mRNA alongside the CDR3 regions of TCRs and BCRs to gain insights into clonotype constitution.
For a deep dive into immune profiling such as isotype and repertoire information. Capturing of poly-adenylated mRNA with barcoding beads. Using our proprietary circularization process, we can enrich the V, D, J, and C regions of the TCR/BCR for full-length sequencing using short read sequencing.
Proudly advancing research with innovative single-cell solutions, Singleron maximizes sample value through high-quality data generation and advanced bioinformatics interpretation. Trusted globally by research and pharma customers, our single-cell analysis service fuels high-impact publications and accelerates scientific discoveries.
Need advice with planning your immune profiling experiments? Not sure which solution is most suitable? We provide free consultation on your project.
Solid tissue will be dissociated in our service lab into a single cell solution.
TCR/BCR libraries | full-length libraries and whole transcriptome libraries are generated according to your research needs.
You will receive a detailed report for every sample including:
If you wish in depth and personal bioinformatics support, we recommend the advanced data analysis package.
Your personal project manager will accompany you all the way through your single cell journey with us, keeping you update on
tissue amount (30 mg is the recommended minimum)
Human (VDJ), Human and Mouse (Full-length)
Captured cells/nuclei
sequencing depth for enriched library | 20 000-50 000 reads/cell for whole transcriptome
2-3 CeleScope reports per sample, 2-6 fastq files per sample, gene count matrix + clonotype/receptor information tables
Start your single cell journey today! Contact us for a free consultation on your next single cell project and learn more about our immune profiling service.