Empowering Quality Cell Input for Quality Data Output. The sCelLiVE® Tissue Dissociation Kit provides solutions for preservation and dissociation of fresh, solid biological samples.
This all-in-one kit contains two ready-to-use solutions for gentle sample preservation for up to 72h and for efficient tissue dissociation.
sCelLiVE® Dissociation Master Mix enzymatically dissociates various samples. This unique solution has been validated on more than 400 sample types from both human and mice.
sCelLiVE® Tissue Preservation Buffer efficiently simulates the physiological environment and ensures high cell viability over a 72-hour time period.
Viability of cells using sCelLiVE® Tissue Preservation Solution with brain tissues collected freshly or stored at 4ºC for 24 and 72 hours. Approximately 90% of the cells from the tissue stored in the Preservation Solution were still alive after 72 hours storage.
Comparison of sCelLiVE® Tissue Dissociation Master Mix to other protocols on dissociation of glioblastoma samples: a homebrew protocol from literature and a commercial product from Supplier M. Cell suspension generated with sCelLiVE® Tissue Dissociation Master Mix had the highest cellular viability. The proprietary formulation does not affect cell viability.
Stable storage and transportation
One solution suitable for a wide rage of different tissue and sample types
Tissue dissociation time Manual: 30min Automation (PythoN): 15min
The kit covers tissue preservation solution and tissue dissociation master mix for 16 samples, each of about 100 mg.
The preservation buffer is optimized for fresh, solid tissue samples. It is not recommended for cell suspensions or organoids.
We have extensively tested our dissociation buffer with various organs of human and mouse origin. However, we have also used it with other species such as zebrafish (Danio rerio).
No. We find that the best solution for cell suspensions is cryopreservation for transportation or storage. Please be sure to check the best cryopreservation medium for your particular type of cells and also please be aware that we cannot guarantee the loss of some subtypes upon freezing.
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