Discover Tensor High Throughput Single Cell & Library Processing System, our high-efficiency liquid handling solution that automates the whole workflow – from single cell partitioning to NGS library generation.
Reproducibility of BI results
30K PBMC per sample were loaded on chip and processed into transcriptomic libraries either with Tensor (n=8) or manually (n=4). The histogram shows the proportion of each respected cell type in Tensor samples (n=8). Here, we demonstrate that the biological diversity of cell types remains consistent and no technical artifact can be observed.
Cross-contamination assessment
To eliminate the possibility of cross-contamination, we performed the entire workflow using 7 mouse HEPA samples and 7 human A549 samples. During both the chip loading and library preparation, samples from different species were placed adjacent to each other. For each cell, their number of detected genes was quantified, and each gene was assigned to either HEPA or A549 cells. The results indicate that human cells rarely contain reads annotated to the mouse genome, and vice versa, demonstrating minimal cross-species contamination.
Tensor only takes cell suspension as input. Samples from tissues need to undergo dissociation beforehand. We recommend a cell viability of at least 70%
No, reusing the chips is not an option. Please always use new chips for your single cells experiments to avoid cross-contamination and ensure best quality of processing and results.
Tensor is designed to be operated by life science lab personnel with minimum robotics knowledge, as the program is very intuitive. The operator would be required to have expertise in tissue preparation only.
The kits are designed to be used in increments of 8 samples per run. While smaple size can be freely choosen, running multiple rounds of small (<4) sample sizes are not recommended, as it might lead to exhaustion of reagents.
Tensor comes with established maintenance programs, both for daily (~min) and weekly
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