Discover the complete transcriptome story with short-read sequencing
See the full picture. MobiuSCOPE Single Cell RNA Sequencing Kit combines 3′ and 5′ RNA-seq approaches in one solution, providing full length transcript insight. Discover splicing variants, SNPs, and fusion genes with the the accuracy, depth, and cost-effectiveness of short-read sequencing.
MobiuSCOPE empowers researchers to extract maximum biological insights from every sample, revealing critical transcript information that traditional 3' or 5' biased methods miss.
Using our proprietary circularization technology, MobiuSCOPE creates both 3′ and 5′ libraries from the same sample, providing unbiased full-length coverage using standard short read sequencing platforms. MobiuSCOPE demonstrates uniform coverage across the entire transcript length, while traditional 3′ scRNAseq and 5′ scRNAseq show significant bias depending on distance from their respective ends.
MobiuSCOPE captures both 3′ and 5′ ends of transcripts, providing complete coverage across the entire length. This reveals splicing variants, SNPs, and fusion genes that would be missed by methods biased toward either end.
No, MobiuSCOPE is designed to work with standard short-read sequencers (150PE), making it accessible to labs with existing Illumina or similar platforms.
MobiuSCOPE provides full-length transcript information at a fraction of the cost of long-read platforms, making comprehensive transcriptome analysis more accessible.
By capturing the full length transcript, MobiuSCOPE improves mapping efficiency for species with incomplete genome annotations.
MobiuSCOPE works with fresh tissue, frozen samples, and cell lines.
Contact us for a quote or assistance in planning your next experiment that requires complete transcript insights.