Empowering Quality Cell Input for Quality Cell Output. The sCelLiVE Flow Tissue Dissociation Kit is optimized for creating single cell suspensions from fresh tissue while keeping their surface epitopes intact for downstream analysis with flow cytometry.
TME cell composition can greatly influence cancer prognosis and the choice of therapy. Here, cancerous tissue from a patient with Lung Adenocarcinoma (LUAD) was extracted and dissociated using sCelLiVE® (flow) in combination with PythoN®, stained with antibodies (n=21), pooled, and further analysed with FACS. The specific cell type can be determined by studying the co-expression of surface markers. Among the non-WBC analysed, it was observed how EpCAM, a tumour marker for cancer cells of epithelial origin, is frequently co-expressed with PD-L1 on, advocating for anti-PD-L1 therapy.
(1) Once knowing the cell composition, and the proportion between epithelial cell and T-cells, one can categorize the tumour into cold tumour (less immune cell, hence poor response to immunotherapy) and hot tumour. (2) By further investigating the cell types between patients with different types of lung cancer, Lung Squamous-cell Carcinoma (LUSC) seem to have a significantly high tissue (epithelial cells) ratio, advocating for a different strategy than immunotherapy. (3) In parallel, one can investigate the T-cell population, to get more insights on the specific identity and their possible role in tumour control or immune evasion.
from sample to library within one workday
The kit contains dissociation solutions for 24 samples. Preservation solution can be purchased additionally.
Yes, the sCelLiVE® (flow) kit can be used both manually and with our PythoN/Junior machines. Please talk to our Sales Team for refilling the dissociation tubes.
The cells can be used for single cell library construction post FACS. We also noted that cell plating works well with this dissociation solution, as it’s more gentle with adhesion molecules.
No, there are no antibodies included in sCelLiVE Flow.
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