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scellive tissue dissociation kit

sCelLiVE® Flow – The Epitope Guardian

Empowering Quality Cell Input for Quality Cell Output. The sCelLiVE Flow Tissue Dissociation Kit is optimized for creating single cell suspensions from fresh tissue while keeping their surface epitopes intact for downstream analysis with flow cytometry.  

sCelLiVE® Flow at a glance

Suitable for flow cytometry and single cell sequencing
Manual or automated tissue dissociation
Universal for all tissue types

Dissociated biopsies from lung cancer patients analyzed by flow cytometry

Figure A:

TME cell composition can greatly influence cancer prognosis and the choice of therapy. Here, cancerous tissue from a patient with Lung Adenocarcinoma (LUAD) was extracted and dissociated using sCelLiVE® (flow) in combination with PythoN®, stained with antibodies (n=21), pooled, and further analysed with FACS. The specific cell type can be determined by studying the co-expression of surface markers. Among the non-WBC analysed, it was observed how EpCAM, a tumour marker for cancer cells of epithelial origin, is frequently co-expressed with PD-L1 on, advocating for anti-PD-L1 therapy. 

Streamlined workflow

from sample to library within one workday

Tissue

Automated or Manual Tissue Dissociation

Single Cell Suspension

Staining

Flow Cytometry Analysis

Ready Flow Data

Applications Areas

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FAQ

The kit contains dissociation solutions for 24 samples. Preservation solution can be purchased additionally. 

Yes, the sCelLiVE® (flow) kit can be used both manually and with our PythoN/Junior machines. Please talk to our Sales Team for refilling the dissociation tubes. 

The cells can be used for single cell library construction post FACS. We also noted that cell plating works well with this dissociation solution, as it’s more gentle with adhesion molecules. 

No, there are no antibodies included in sCelLiVE Flow.

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