Ready for your all-in-one single cell sequencing solution?
dynascope singleron

DynaSCOPE®- Time-Resolved Transcriptomics at Single Cell Level

The DynaSCOPE® Single Cell RNA Dynamic Library Kit employs chemical labeling to discriminate nascent mRNA allowing to distinguishing newly transcribed and pre-existing RNA within single cells.

Kit Highlights

Discover transcriptome dynamics at single cell level
Suitable for in vitro and in vivo experiments
Based on S4U labelling technology

The accurate measurement of freshly synthesized and long-lasting mRNA might help to understand immediate regulatory dynamics in tissue as well as developmental, environmental changes, drug treatments and infection mechanisms from an experimental and bioinformatical perspective.

DynaSCOPE Technology

During the DynaSCOPE workflow, a nucleotide analogue (uracil-analogue) is incorporated into freshly synthesized transcripts during transcription, but not into static transcripts. The incorporated uracil-analogue is converted into cytosine analogue and later recognized as cytosine (C), while the unlabeled uracil in stable transcripts is converted into thymine (T). Nascent and long-lasting transcripts can then be differentiated by bioinformatic analysis provided by the CeleScope® software.

In Vivo Application of DynaSCOPE®

(A) Two mice at different ages were abdominally injected with labelling reagent followed by DynaSCOPE library preparation. (B) Cell and sample type annotation are shown in the UMAP plot .  (C) UMAP plot shows transcriptionally active cell clusters with shades of red indicating the transcriptional synthesis rate.

Streamlined workflow: from sample to library within one workday

In vitro | In Vivo labelling S4U labelling

Single Cell Suspension

Cell Partitioning, Lysis & Barcoding

Library Generation

Sequencing

Bioinformatics Analysis

Applications Areas

Technical Specifications

In vivo | In vitro

S4U labelling can be performed in vitro or in vivo

Manual

Full instruments-free workflow

Automated

PythoN: tissue dissociation
NEO: single cell processing

0.5-4h labelling

Suitable labelling time depends on the tissue used

30 000 cells

choose between SD and HD chip depending on required capture rate

Downloads

FAQ

The selection of labeling time to effectively distinguish RNA synthesis rates under various conditions is contingent upon sample types and the inherent RNA synthesis rate in different cells. We recommend to incubate cells for a duration ranging between 0.5 and 4 hours. For most sample types, we typically recommend a standard duration of 2 hours.

We advise to utilize DMEM medium supplemented with 20% FBS, for optimal metabolic labeling,

The suggested total number of cells for metabolic labeling is in the range of 1 to 2 million (1-2 × 10^6).

The effect on cell viability varies with cell types; some cells may experience a 5-10% decrease in viability after culture. It is imperative to maintain high cell viability, aiming for approximately 80%. The total number of living cells should exceed 40,000 after incubation for subsequent steps.

Get a Quote Now

Get a Quote Now

Start your single cell journey today!
Contact us for a quote or assistance in planning your next experiment.