Plants are masters of compartmentalization. From the intricate layers of the root apical meristem to the specialized cells within a leaf’s stomatal complex, function is dictated by cellular identity. Single cell sequencing is a transformative tool for uncovering the fundamental mechanics of plant growth, resilience, and evolution. This leads the way towards understanding cellular diversity of plants which is essential for addressing global challenges in food security, climate resilience, and sustainable agriculture.
Plant tissues present unique structural challenges such as the rigid cell wall and large vacuoles containing debris. These are overcome through innovative workflows for single cell and single nucleus transcriptomics. Nowadays, the resolution needed to map cell type-specific development and environmental responses can be achieved at scale.
The primary challenge in plant single-cell transcriptomics is the rigid cell wall. Traditionally, researchers extracted protoplasts for RNA isolation. However, single-nucleus sequencing (snRNA-seq) has rapidly become the preferred method for several reasons:
However, if you still wish to perform protoplast extraction because the cytoplasmic RNA is important or you need maximum transcript detection, please contact us and our Tech Support Team will discuss your project’s details and feasibility.
PythoNi and PythoN Jr. are automated tissue dissociators that allow to perform automated single nucleus extraction. Programs are highly adjustable for a wide range of parameters including temperature, rotation speed, cycle number, heating duration, and orientation.
Whether you prefer to perform your experiments in-house using our kits or choose to have Singleron’s expert staff manage the intricate hands-on work in our specialized laboratories, we have you covered.
We support you at every step of your journey. From initial tissue selection to final data interpretation, our team provides the technical and biological consultation needed to ensure your project’s success.
Plant tissues vary significantly between species and developmental stages. Our specialists consult with you to determine the best approach—tailoring the protocol to your specific model organism, from Arabidopsis and moss to complex woody species like poplar.
Spatial Transcriptome of Maize Ear Development
Mechanisms of Wood Plasticity and Drought Tolerance in Poplar
Single cell sequencing of plants: a growing story
Single-Cell Decoding of Cellular Lineages and the Evolution of Plant Exceptional Traits
Are you planning a project with plant tissue? We’re rooting for you!