GEXSCOPE® Single Cell RNA Library Kit GEXSCOPE® Single Nucleus RNA Library Kit GEXSCOPE® Microbial Single Cell RNA Library Kit HD (Yeast) GEXSCOPE® Single Cell V(D)J Kit DynaSCOPE® Single Cell Dynamics RNA Library Kit FocuSCOPE® Single Cell Targeted Capture Kit sCircle® Single Cell Full Length Immunoreceptor Library Kit CLindex® Sample Multiplexing Kit sCelLiVE® Tissue to Living Single Cell Suspension Kit AccuraCode® RNA Library Construction Kit
Singleron PythoN Junior™
The Singleron PythoN Junior™ Automated Tissue Dissociation System is a compact and flexible instrument specifically designed to streamline the dissociation process from solid tissue to high-quality single cell suspension. Equipped with broad-spectrum sCelLiVE Tissue Dissociation Mix and disposable Singleron PythoN Dissociation Units, the automated system combines mechanical treatment and enzymatic digestion for a fast and efficient dissociation of fresh tissue samples.
Singleron PythoN Junior™ dissociates tissues with high reproducibility.
Murine lung tissues were processed in four replicate experiments using Singleron PythoN Junior™. Single cell suspensions with >85% cell activity were obtained. Cell yield and viability were consistent across the four replicates, showing the high stability and reproducibility of the Singleron PythoN Junior™ Automated Tissue Dissociation System.
Singleron PythoN Junior™ is suitable for low-input samples.
In clinical settings, in order to detect the pathological characteristics of specific tissues, needle biopsy is often required. The low weight of the obtained needle biopsies is often a challenge for single-cell sequencing workflow. Here, we used Singleron PythoN Junior™ to process 10 mg and 60 mg mouse spleen tissues, and achieved high cell viability and yield for both samples.
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