Obtain comprehensive single cell mRNA and non-coding RNA insights from formalin-fixed paraffin-embedded (FFPE) tissues.
Current FFPE solutions rely on pre-defined gene panels and simplified gene-counting methods, missing non-coding RNAs and full-length transcript information. Now, with Singleron’s optimized random priming strategy, you no longer have to compromise your data collection.
Whether the goal is to explore more conditions, capture biological diversity, or generate enough data for your AI model, multiplexing lets you expand across samples, giving you richer datasets without simply sequencing deeper.
With our multiplex solution for RetroSCOPE®, up to 10 samples can be loaded per chip, capturing up to 200,000 cells in a single library reaction.
Figure 1: Mouse bladder cancer FFPE single-nucleus sequencing data clustered by gene expression and overlaid with non-coding RNA expression. Even when an ncRNA’s exact function remains unknown, its expression can serve as a cell-type marker in reference atlases. Furthermore, cell-type–associated ncRNA patterns offer a powerful window into cellular identity and biology.
Low quality samples were recoverable using RetroSCOPE® FFPE solutions, providing high-quality data from compromised samples.
Figure 3: Mouse thymus nuclei were labeled with 10 distinct indices using the RetroSCOPE Multiplexing Workflow.
Gain insights directly from our founder, Dr. Nan Fang as she presents the latest data from our FFPE solution.
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