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We are out there: Singleron publications highlights 2022

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We are out there: Singleron publications highlights 2022

In 2022, Singleron passed the magic number of 100 publications using our technology. So, yes: we are definitely out there and will keep coming. The end of the year is always a time for reflection and New Year’s resolutions, a season of looking back and forth. Looking back, we would like to highlight some of our most relevant publications.

Single-cell RNA-seq of UVB-radiated skin reveals landscape of photoaging-related inflammation and protection by vitamin D (Lin et al., 2022)

Even in winter, we need to protect our skin from ultraviolet light. Researchers used Singleron single-cell sequencing technology to investigate UV-irradiated mouse skin. They found fibroblast inflammation, demonstrating differential gene expression and revealing the significance of macrophages in tissue repair and vitamin D-induced inhibitory effect on skin inflammation.

Improved ClickTags enable live-cell barcoding for highly multiplexed single cell sequencing (Zhao et al., 2022)

Clindex

Science is a grand whole. Even in our times of extreme specialization, discoveries from one field are applied to another, creating interdisciplinary wonders. Like in this work where the authors use Click chemistry technology (awarded this year with the Chemistry Nobel Prize) to barcode cells, providing a new strategy for sample multiplexing in single-cell RNA-seq.

Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques (Zheng et al., 2022)

COVID-19 appeared three years ago, and it seems it is here to stay. Singleron technology elucidates this virus’ mechanisms of action and its consequences. Pathological and immune responses to COVID-19 display different lung and intestine dynamics. In this work, the researchers created a transcriptional atlas of longitudinally collected lung and intestinal tissue samples from SARS- CoV-2-infected monkeys, mapping the differences in gene expression. Using our GEXSCOPE Single Cell RNA-seq Library Kit, the researchers found an imbalanced immune dynamic in the intestinal mucosa during SARS-CoV-2 infection. This may underlie ongoing rectal viral shedding and mild tissue damage.

Reflecting on the past year’s success and looking forward, we are optimistic that next year will be at least as great as the one we leave behind, if not better!

References

Lin, Y., Cao, Z., Lyu, T., Kong, T., Zhang, Q., Wu, K., Wang, Y., & Zheng, J. (2022). Single-cell RNA-seq of UVB-radiated skin reveals landscape of photoaging-related inflammation and protection by vitamin D. Gene, 831, 146563. https://doi.org/10.1016/j.gene.2022.146563

Zhao, X.-L., Sun, S., Yu, W., Zhu, W., Zhao, Z., Zhou, Y., Ding, X., Fang, N., Yang, R., & Li, J. P. (2022). Improved ClickTags enable live-cell barcoding for highly multiplexed single cell sequencing. RSC Chemical Biology, 3(8), 1052–1060. https://doi.org/10.1039/D2CB00046F

Zheng, H., Chen, Y., Li, J., Li, H., Zhao, X., Li, J., Yang, F., Li, Y., Liu, C., Qin, L., Zuo, Y., Zhang, Q., He, Z., Shi, H., Li, Q., & Liu, L. (2022). Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques. Cell Reports, 39(8), 110864. https://doi.org/10.1016/j.celrep.2022.110864

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