Dissecting the tumor immune microenvironment and CAR-T cell therapy resistance – Dr. Maxine LamWatch nowGlioblastoma (GBM) remains one of the most lethal malignancies with limited treatment options and dismal prognoses. Immunotherapy, particularly chimeric antigen receptor (CAR)-T cell therapy, has emerged as a promising avenue for GBM treatment. However, the complex tumour immune microenvironment and the presence of the blood-brain barrier (BBB) present formidable challenges to the efficacy of CAR-T cell therapy. We present a novel in vitro model of GBM that incorporates a functional BBB, aiming to replicate the physiological barriers encountered by CAR-T cells in GBM patients.Utilizing this model, we investigated the efficacy of CAR-T therapy against GBM and identified a subset of GBM cells resistant to CAR-T treatment. By employing single-cell analysis, we delineated the dynamic changes occurring within the various cell types in the tumour microenvironment. We identify alterations in cell states in T cell phenotypes, GBM cell populations and the BBB after CAR-T therapy.Our findings shed light on the complexities underlying CAR-T immunotherapy for GBM and highlight the importance of developing strategies to overcome resistance mechanisms within the tumour microenvironment. This physiologically relevant model provides a valuable platform for preclinical testing and the optimization of CAR-T cell therapies, by enabling accurate and highly controlled in vitro experiments. Ultimately this will bring us closer to improved treatment strategies for this devastating disease. About the keynote speakerDr Maxine Lam earned her doctorate in Developmental and Stem Cell Biology from the MRC LMCB in London as a Wellcome Trust and A*STAR PhD fellow. Transitioning to cancer biology, she briefly worked at the Institute of Cancer Research before joining IMCB in 2020. Dr Lam received the Career Development Fund by A*STAR to develop an in vitro microphysiological model for glioblastoma and the blood-brain barrier. Collaborating across disciplines, she employs interdisciplinary approaches to unravel the complexities of brain cancers and advance novel perspectives in the field. This webinar is co-organized by Singleron, Azenta, and RSC, A*STARThis might interest you as well BlogUnlocking the Power of Precision Medicine: An Introduction to CAR-T Single Cell Analysis More info ProductGet the pole position in the CAR-T race: How Single Cell Multi-Omics can accelerate precision medicine More infoCheck out our latests webinars Learn more Single Cell, Infinite Insights: Overcoming Obstacles for Top-Notch Data In this webinar, you will embark upon a research journey alongside Luigina De Leo and our other speakers, using a challenging case study focusing on… Read more Uncover Cellular Heterogeneity with Advanced Single-cell Multi-Omics Approaches Join us as we delve into the latest single cell sequencing technology advancements, offering deeper insights into complex biological systems. Our webinar will explore the… Read more Single-cell Multi-omics for Immuno-oncology - Prof. Fan Rong Prof. Fan Rong, the Harold Hodgkinson Professor of Biomedical Engineering and Pathology at Yale University, has been driving single-cell innovation since 2006. With expertise in… Read more
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