How long do I need to perform the metabolic labeling?

The metabolic labeling must be adjusted for different samples. The recommended labeling time is around 0.5-4h based on the transcriptional activity of the cells. We suggest starting with an incubation time of 2 hours and adjusting accordingly. Increasing the labeling time beyond 4 hours might help to ensure a good conversion rate, but it might be detrimental to the health of the animal or the quality of the cells.