Is it possible to re-amplify the cDNA in case it is low yield?Home FAQ QC criteria cDNA QC criteria Is it possible to re-amplify the cDNA in case it is low yield?We do not recommend re-amplifying the cDNA for library preparation and sequencing, since this might increase the PCR bias towards more abundant transcripts. An exception to this is the cDNA used for targeted or enriched products, such as in sCircle Single Cell Full-Length TCR/BCR Kit, where the cDNA amount needed to produce the receptor libraries is much higher.