Is it possible to use a different buffer (other than PBS) for cell loading?Home FAQ Technology SCOPE-chip Is it possible to use a different buffer (other than PBS) for cell loading?As our chip is an open system, it allows some changes to meet the needs of each experiment. In this regard, a different buffer can be used (for example W5 buffer for protoplasts) when it doesn’t contain metal chelator agents that might interfere with the reverse transcription (such as EDTA), and it must be RNAse free. In this case, we recommend following these steps:Wash the chip with your own buffer prior to cell loading.Load the cells, which are in suspension in your own buffer.Wash the chip with PBS to get rid of the excess cells that are not within microwells, following the handbook instructions.Load the beads (resuspended in 60ul of PBS), following the handbook instructions.Add the lysis buffer and continue with the instructions as described in the handbook.