I am planning a large-scale single cell study. What sample multiplexing solutions does Singleron offer?

Singleron offers two sample multiplexing solutions tailored to different sample types:

1. CLindex^® Sample Multiplexing Kit – For live cell suspensions.
2. RetroSCOPE^® Multiplexing RT Kit – For FFPE samples.

1. CLindex Sample Multiplexing Kit

The CLindex Sample Multiplexing Kit uses click chemistry to label cell surfaces with modified oligonucleotides in a cell type-agnostic manner. It supports multiplexing of up to 96 samples, allowing multiple samples to be pooled and processed together on the SCOPE-chip for single cell capture.

This approach is particularly suitable for large-scale studies involving multiple experimental conditions, such as CRISPR-based perturbation screens using cell lines.

2. RetroSCOPE Multiplexing RT Kit

The RetroSCOPE Multiplexing RT Kit introduces sample-specific barcodes during the in-situ reverse transcription step that occurs within intact nuclei. The nuclei of multiple samples can subsequently be pooled and processed together on the same SCOPE-chip for single cell capture.

This approach is particularly suitable for large-scale studies involving FFPE samples.

Alternatively, SNP-based demultiplexing (label-free approach) can be used to distinguish cells originating from genetically distinct donors without requiring additional sample-labeling reagents.

Cells from different donors can be pooled and loaded onto the same SCOPE-chip for single cell capture. Following sequencing, naturally occurring single-nucleotide polymorphisms (SNPs) are used to assign individual cells to their respective donors. Known donor SNP profiles can facilitate more accurate sample assignments.

This approach is particularly useful for large-scale studies involving samples from multiple genetically distinct donors.

For more information, see the Singleron webinar: Label-Free Multiplexing Using SNP Genotyping and Bioinformatics.