How do I select the most suitable single cell analysis workflow for my sample type?

Singleron offers different single cell analysis workflows to accommodate samples with different preservation methods and processing requirements.

• Single cell workflows: Suitable for fresh samples and cryopreserved cells with intact cell membranes to retain intracellular RNA. If fresh tissue cannot be processed immediately, the sCelLiVE^® Tissue Preservation Buffer can help preserve the tissue until processing. For low-abundance cell populations, FACS or MACS can be used for enrichment before processing. SCOPE-chips® are compatible with both FACS- and MACS-sorted cells. To minimize sorting-related stress, enriched cells should be processed immediately.
• Single nucleus workflows: Suitable for snap-frozen tissues, where cell membrane integrity may be compromised during freezing and storage.
• FFPE workflows: RetroSCOPE^® is designed for gene expression analysis of formalin-fixed, paraffin-embedded (FFPE) samples, accounting for RNA degradation associated with fixation and storage.

For guidance on selecting the most suitable workflow for the sample and experimental needs, please contact the Singleron team.