How can I customize a PythoN i or PythoN Junior tissue dissociation program tailored to my sample type?

PythoN i^TM and PythoN Junior^® offer preset and customizable programs for different tissue types and downstream applications. To tailor a tissue dissociation program to the sample:

1. Start with a recommended program. Choose the preset program for the tissue type, or ask Singleron’s technical support team to recommend a program and reagents. Use these recommendations as a starting point.
2. Monitor the dissociation. For challenging tissues, take an aliquot and check it under the microscope every 5–10 minutes. The goal is to achieve efficient cell release while maintaining cell viability and minimizing undissociated tissue fragments.
3. Adjust the parameters. Based on the quality of the resulting single cell suspension, adjust parameters such as rotation speed, rotation duration and direction, and number of repetitions to optimize the workflow.
4. Save the program. Save the optimized program on PythoN i or PythoN Junior and select it for future runs, so results stay consistent and reproducible.

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