How can I detect mutations or fusion genes in single cell RNA-seq?Home FAQ Single Cell Kits FocuSCOPE Single Cell RNA Library Kit How can I detect mutations or fusion genes in single cell RNA-seq?Yes, Singleron’s FocuSCOPE® technology enables the detection of mutations and gene fusions alongside gene expression profiling in the same single cells.FocuSCOPE uses barcode beads containing both poly(dT) oligonucleotides to capture polyadenylated mRNA and target-specific probes to capture selected RNA regions, such as mutation sites, gene fusion junctions, and intracellular viral or bacterial RNA sequences.The target-specific probes enhance the sensitivity of detecting these regions and enable the capture of sequences located far from the 3′ end of transcripts, which are typically difficult to detect using conventional 3′ single cell RNA sequencing methods.This approach allows researchers to simultaneously characterize gene expression profiles and detect specific genetic alterations or pathogen-derived RNA within individual cells.