I want to detect neutrophils in my single cell RNA-seq experiment. Is this possible with Singleron?

Yes, neutrophils can be profiled with Singleron GEXSCOPE^® Single Cell RNA Library Kit.  Singleron SCOPE-chip^® allows cells to gently settle into individual microwells, and is well suited for sensitive cell types such as neutrophils.

Studies using Singleron GEXSCOPE Single Cell RNA Library Kit have captured neutrophils from fresh whole blood (Zhang et al., 2023), and from lung cancer needle biopsies stored in Singleron sCelLiVE^® Tissue Preservation Solution (Wu et al., 2021).

Upstream sample processing is crucial as neutrophils are fragile and have low RNA content. A few considerations to note for sample preparation:

• Immediate processing of fresh samples is highly recommended.
• Avoid prolonged incubation on ice. Neutrophils prefer room temperature (Kuhns et al., 2015).
• For whole blood samples, always process fresh and deplete red blood cells using conventional red blood cell lysis buffers. Proceed immediately to single cell assays. Do not use cryopreserved samples.

Related the application note: A Solution for Robust and Reliable Single Cell RNA-Sequencing of Neutrophils

References:

Wu F, et al. Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer. Nat Commun. 2021; 12:2540. doi:10.1038/s41467-021-22801-0
Zhang Y, et al. A single-cell atlas of the peripheral immune response in patients with influenza A virus infection. iScience. 2023;26(12):108507. doi:10.1016/j.isci.2023.108507

Kuhns DB, Long Priel DA, Chu J, Zarember KA. Isolation and functional analysis of human neutrophils. Current Protocols in Immunology. 2015;111:7.23.1–7.23.16. doi: 10.1002/0471142735.im0723s111