What is covered by Singleron’s single cell sequencing service?

Singleron’s single cell sequencing service covers the end-to-end workflow from sample to data. A project includes the following steps:

1. Technical consultation
Each project begins with a consultation to review experimental design, sample requirements, and key considerations for single cell or single nucleus sequencing. Our scientists answer questions specific to your sample type and research goals.

2. Tissue preservation (optional)
For solid tissue samples, tissue can be stored in Singleron’s sCelLiVE^® Tissue Preservation Solution without fixation for up to 72 hours at 4 °C before processing. This is particularly suitable for samples that need to be stored for shipment or overnight.

3. Tissue dissociation (optional)
Solid tissue can be dissociated into a single cell or single nucleus suspension using Singleron’s PythoN^® tissue dissociation system. The system has been validated across a wide range of species and tissue types, including skin, tumor, and organoid samples. Contact us to confirm compatibility or to obtain an existing protocol for your tissue type.

4. Library construction and sequencing
Libraries are prepared with Singleron’s GEXSCOPE^® single cell RNA sequencing kits. In addition to standard single cell and single nucleus transcriptome profiling, the following applications are available:

• Targeted detection of mutations (including single nucleotide variants) and of viral and bacterial transcripts
• Full-length single cell RNA sequencing for analysis of alternative splicing, mutations, and fusion genes
• Single cell Iimmune profiling (full-length TCR/BCR sequencing)
• Single cell RNA sequencing of yeast
• RNA dynamics profiling by metabolic labeling of nascent RNA with 4-thiouridine (S4U)
• Single cell ATAC-seq (chromatin accessibility)
• Single cell glycosylation profiling
• Joint single cell genome and transcriptome analysis
• Sample multiplexing
• Single nucleus RNA sequencing of FFPE samples
• Coding and non-coding RNA sequencing
• Single cell CRISPR screening

5. Data analysis
With a Singleron single cell sequencing service project, you receive:

– FASTQ data files

– QC reports

– Gene expression count matrices

The matrix files are compatible with major data analysis pipelines.

In addition, data can be analyzed with Singleron’s CeleLens^® Cloud, a code-free analysis software, which requires no bioinformatics experience. For customized or more advanced analyses, Singleron’s bioinformatics team also offers analysis services.

To discuss your project, please contact us.