Single cell analysis has never been sweeter. The ProMoSCOPE™ Single Cell Glycosylation Detection Kit utilizes specific tags for the simultaneous detection and quantification of cell surface glycosylation and the entire transcriptome at a single-cell resolution.
The ProMoSCOPE-Tag technology is designed with chemical moieties for specific recognition and covalent binding to N-Acetyllactosamine (LacNAc) on cell surface proteins. This tag includes a cell barcode and poly A for seamless processing with the entire transcriptome. After labeling, partitioning, and cell lysis, mRNA and ProMoSCOPE Tags bind to barcoding beads, and reverse transcription prepares ProMoSCOPE and total transcriptome libraries.
Mouse bone marrow was analyzed with ProMoSCOPE and whole transcriptome single cell sequencing. Pearson correlation coefficient (r = 0.99) indicates a robust positive linear relationship between labeled and non-labeled groups in the context of whole RNA single-cell transcriptome and cell clustering, with no impact of labeling status on the overall transcriptomic landscape.
Mouse bone marrow single cell whole transcriptome and glycosylation patterns were analyzed using the ProMoSCOPE Kit. (A) Cell types were annotated and subjected to (B) TAG UMI plotting to visualize glycosylation status. (C) Cell type dependent UMI counts.
Neutrophil activation markers and proliferation markers along with other genes involved in proliferation.
lymphocyte differentiation, activation, and migration
Glycosylation and whole transcriptome from the same single cells
Full instruments-free workflow
PythoN: tissue dissociation NEO: single nuclei processing
The entire workflow from sample to two libraries can be done in one workday
capturing rate with SD chip configuration
ProMoSCOPE technology uses chemoenzymatic method where ProMoSCOPE Tag covalently binds to N-Acetyllactosamine (LacNAc) which is found in most glycan structures.
This product has been tested with a variety of human and mouse cells. Theoretically, the labeling reagent are also suitable for other species.
We recommend 3000 reads /cell for ProMoSCOPE library.
Yes, combination of PromoSCOPE and CLindex kits works well.
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