ProMoSCOPE Single Cell Glycosylation Detection Kit

Glycosylation is an enzymatic process in which carbohydrate groups are attached to the protein or lipids’ backbone. Protein glycosylation is one of the most common post-translational modifications and can be seen in all living organisms. It influences processes such as protein folding, stability and membrane organization. ProMoSCOPE Single Cell Glycosylation Detection Kits enable accurate quantification of cell surface glycosylation level together with gene expression profiles in single cell level simultaneously. It is achieved by chemoenzymatic method where ProMoSCOPE tag covalently binds to N-Acetyllactosamine (LacNAc) at cell surface. The level of glycosylation together with whole transcriptome information can be used for in-depth analysis of cell status and functions in complex biological systems.

ProMoSCOPE Single Cell Glycosylation Detection Kit uses the SCOPE-chip^®, a portable, microfluidic chip with microwells that integrates multiple steps of the processing workflow such as single-cell capture, cell lysis and capture of cellular mRNA. Sequencing ready libraries can be prepared with the reagents included. The SCOPE-chip^® is easy to use and can be operated manually without special equipment. It is suitable for cells from a large variety of tissue types. 

ProMoSCOPE labeling has no effect on transcriptome

Thymus from mice are extracted and both labeled and unlabeled single cell suspensions are loaded to SCOPE-chip^® and processed by ProMoSCOPE Single Cell Glycosylation Detection Kit. Cells are annotated in the UMAP plot and shown in (A). UMAP plot colored by treatment is shown in (B). No difference has been observed in the cell type composition (C) Correlation between control and treated samples is presented in (D) demonstrating that there is no obvious effect of labelling on transcriptome.

Cell specific glycosylation abundance in bone marrow

Bones from mice are extracted and both labeled and unlabeled single cell suspensions are loaded to SCOPE-chip^® and processed by ProMoSCOPE Single Cell Glycosylation Detection Kit. Cells are annotated in the UMAP plot and shown in (A). UMAP plot colored by number of UMI counts (B) and violin plot of UMI counts (C) are presented which uncovers cell specific glycosylation abundance in bone marrow.

Application areas