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What are the differences between PythoN i and PythoN Junior, and how do I choose the right instrument for tissue dissociation?

Singleron’s PythoN i and PythoN Junior automate tissue processing to generate single cell suspensions, single nucleus suspensions, and tissue homogenates. Both instruments feature independently operating channels, allowing different samples to be processed simultaneously using different programs in the same run. PythoN Junior accommodates up to two samples per run in a compact footprint, while PythoN i processes up to eight samples per run and offers a larger selection of pre-set and customizable programs. The best choice depends on your sample throughput, available bench space, and intended application.

FeaturePythoN i™PythoN Junior®
Sample throughputUp to 8 samples per runUp to 2 samples per run
Sample input10–1000 mg
Independent channelsYes
Dimensions36 × 23 × 21 cm16.3 × 15.2 × 25.3 cm
Standard run time~15 min, depending on the sample
Pre-set programs9010
Customizable programsUp to 500Up to 90
Multi-step programsYes
Customizable parametersTemperature
Rotation speed and direction
Cycle number
ApplicationsTissue dissociation
Nuclei isolation
Tissue homogenization
Compatible kitssCelLiVE Tissue Dissociation Kit – PythoN® Series (Universal, Skin, and Flow)
Singleron PythoN® Series Nuclei Isolation Kit

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