What are the differences between PythoN i and PythoN Junior, and how do I choose the right instrument for tissue dissociation?Home FAQ Instruments PythoN and PythoN Junior What are the differences between PythoN i and PythoN Junior, and how do I choose the right instrument for tissue dissociation?Singleron’s PythoN i and PythoN Junior automate tissue processing to generate single cell suspensions, single nucleus suspensions, and tissue homogenates. Both instruments feature independently operating channels, allowing different samples to be processed simultaneously using different programs in the same run. PythoN Junior accommodates up to two samples per run in a compact footprint, while PythoN i processes up to eight samples per run and offers a larger selection of pre-set and customizable programs. The best choice depends on your sample throughput, available bench space, and intended application. FeaturePythoN i™PythoN Junior®Sample throughputUp to 8 samples per runUp to 2 samples per runSample input10–1000 mgIndependent channelsYesDimensions36 × 23 × 21 cm16.3 × 15.2 × 25.3 cmStandard run time~15 min, depending on the samplePre-set programs9010Customizable programsUp to 500Up to 90Multi-step programsYesCustomizable parametersTemperatureRotation speed and directionCycle numberApplicationsTissue dissociationNuclei isolationTissue homogenizationCompatible kitssCelLiVE Tissue Dissociation Kit – PythoN® Series (Universal, Skin, and Flow)Singleron PythoN® Series Nuclei Isolation Kit