Can I get alternative splicing information from single cell RNA-seq?Home FAQ Single Cell Kits FocuSCOPE Single Cell RNA Library Kit Can I get alternative splicing information from single cell RNA-seq?Yes, alternative splicing information can be obtained with these Singleron solutions using standard short-read sequencing:Transcriptome-wide, unbiased profiling using Singleron MobiuSCOPE for fresh or cryopreserved samples, and RetroSCOPE® for FFPE or fixed tissues.Target-specific profiling using FocuSCOPE® for fresh or cryopreserved samples.MobiuSCOPE Single Cell Full Length RNA Library Kit generates both 3’ and 5’ libraries from the same cDNA. This provides full-length gene coverage and supports alternative splicing analysis.RetroSCOPE FFPE Single Nucleus Kit uses random priming to capture regions across the transcripts. This enables capture of alternative splicing information even in partially degraded RNA commonly found in FFPE samples.FocuSCOPE uses customised probes designed against specific splice isoforms, thereby enabling targeted and sensitive detection of specific isoforms.