PythoNi is intelligent and versatile.
This system comes with eight channels for sample preparation, with independent parameter selection for different sample types.
"I reached out to Singleron to enquire about single nuclei extraction for my sequencing studies after struggling with various protocols and generating very low quality/low yield samples. The customer support was amazing, from start to finish. Everyone I dealt with in the company went above and beyond to help me. I was able to loan their PythoNi tissue dissociation machine and received online technical support to help me with my specific tissues of interest. With a very straight forward protocol and specific nuclei extraction buffers the machine generated high quality single nuclei from hard to dissociate tissues, such as skeletal muscle and heart. The equipment was very user friendly and intuitive to use and allowed me to process numerous samples at a time, with a single sample taking less than 15 minutes. Using the PythoNi system allowed me to complete a technically difficult project, using a consistent protocol across numerous tissues." – Ami Ketley, University of Nottingham
Efficient tissue dissociation of up to eight different sample types, in parallel or independently. Complete your workflow from sample to library in one work day.
Singleron PythoN i™ was used to process liver, lung, heart, brain tissues, and more, resulting in high-quality single cell suspensions with high cell viability and cell yield.
Fast and efficient tissue homogenization to facilitate extraction of DNA, RNA, Protein, and more.
PythoN i™ has an adjustable temperature, rotation speed, cycle number, heating duration, and orientation, which can be individually defined by the user.
sCelLiVE® Tissue Dissociation Kit Preservation and dissociation of fresh, solid biological samples
Disposable dissociation units for PythoN i™
8-channel tissue dissociation with PythoN i
The PythoN i comes preloaded with some recommended programs. Additionally, we tested several suggested programs for different tissue types.
Yes, aside from tissue samples, we have experience and dedicated programs for some organoids. Depending on the nature of the sample optimization may be required.
The PythoN i tissue dissociation tubes are designed to be single-use only. We cannot guarantee the integrity of the tubes if they are used more than once. Additionally, cross-contamination could occur if the tubes are reused.
Start your tissue dissociation journey today! Contact us for a quote or assistance in planning your next experiment.