Can I use FACS-sorted nuclei for single nucleus RNA sequencing?Home FAQ Single Cell Kits GEXSCOPE Single Nucleus RNA Library Kit Can I use FACS-sorted nuclei for single nucleus RNA sequencing?Yes, FACS-sorted nuclei can be used as input for the GEXSCOPE® Single Nucleus RNA Library Kit; Singleron has processed FACS-sorted nuclei in such a workflow.After sorting, the nuclei should be recounted afterwards and their integrity checked under a microscope before loading onto the SCOPE-chip.