How can I reduce debris for single nucleus RNA sequencing?Home FAQ Single Cell Kits GEXSCOPE Single Nucleus RNA Library Kit How can I reduce debris for single nucleus RNA sequencing?The nuclei suspension should be filtered through a 30 µm cell strainer, then a cleanup step suited to the tissue should be added.For example, for brain samples, debris is most likely myelin, and bead-based myelin removal reagents can be used.Commercial debris removal kits or density gradients such as Percoll®, Ficoll®, OptiPrep™ or a modified sucrose gradient can also help. Debris can be cellular or acellular (for example, extracellular matrix).Debris occupy microwells on the Singleron SCOPE-chip® and adds background to the results, so removing it improves data quality.Contact the Singleron technical team for a nuclei isolation protocol tailored to your tissue.