Can I perform single cell sequencing on frozen samples?

Snap-frozen samples can be analyzed using single nucleus sequencing.

For tissues that have been snap-frozen without cryoprotectants, single nucleus RNA sequencing (snRNA-seq) is generally recommended, as freezing can compromise cell membrane integrity and cell viability. Singleron GEXSCOPE^® Single Nucleus RNA Library Kit supports this application.  

Singleron GEXSCOPE Single Nucleus RNA Library Kit is also well suited for studies where cells with large or irregular shapes are of interest, such as cardiomyocytes and neurons, as well as tissues that are difficult to dissociate into viable single cell suspensions.